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mutant rna probes  (Sangon Biotech)

 
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    Structured Review

    Sangon Biotech mutant rna probes
    Mutant Rna Probes, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+rna+probes/mutant+probes/pm41121296-71-4-13
    Average 86 stars, based on 1 article reviews
    mutant rna probes - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome.
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..

    Mutagenesis:

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome.
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..

    Synthesized:

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome.
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..

    Article Title: Targeted modification of cis-elements in the CUL3 gene to restore exon 9 inclusion for treating Gordon syndrome
    Article Snippet: .. CY5.5 fluorescence-labelled wild-type and mutant RNA probes (WT-RNA and Mut-RNA) were synthesized by Sangon Biotech. ..



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    Image Search Results


    TTP interacts with ARE3 within the LATS2 mRNA 3′-UTR. A, RNP immunoprecipitation assay. 293-EBNA cells were cotransfected with pcDNA6/V5-TTP and a psiCHECK luciferase reporter construct containing ARE3 and ARE4 (Oligo-ARE-w3/w4). A psiCHECK luciferase reporter construct containing mutant ARE3 (Oligo-ARE-m3/w4) was used as a negative control. At 24 h after transfection, the RNP complexes containing TTP were immunoprecipitated with protein G-agarose and anti-V5 or control antibody. The luciferase mRNA in the immunoprecipitates was amplified by RT-PCR. The presence of TTP in the immunoprecipitates was detected by Western blotting with anti-TTP antibody. B and C, RNA EMSA. An RNA EMSA was performed by mixing cytoplasmic extracts containing 4 μg of total protein from pcDNA6/V5-TTP-transfected 293-EBNA cells (B) or A549 cells (C) with 20 fmol of biotinylated wild-type (Wt; ARE-3w/4w) or mutant (Mut; ARE-3m/4w) probe. Anti-V5 (B), anti-TTP (C), or control antibody (Ab) was added to the reaction mixtures. The binding reactions were then separated by electrophoresis on a 5% polyacrylamide gel under nondenaturing conditions. Arrows indicate the position of the TTP-containing band.

    Journal: The Journal of Biological Chemistry

    Article Title: Stability of the LATS2 Tumor Suppressor Gene Is Regulated by Tristetraprolin *

    doi: 10.1074/jbc.M109.094235

    Figure Lengend Snippet: TTP interacts with ARE3 within the LATS2 mRNA 3′-UTR. A, RNP immunoprecipitation assay. 293-EBNA cells were cotransfected with pcDNA6/V5-TTP and a psiCHECK luciferase reporter construct containing ARE3 and ARE4 (Oligo-ARE-w3/w4). A psiCHECK luciferase reporter construct containing mutant ARE3 (Oligo-ARE-m3/w4) was used as a negative control. At 24 h after transfection, the RNP complexes containing TTP were immunoprecipitated with protein G-agarose and anti-V5 or control antibody. The luciferase mRNA in the immunoprecipitates was amplified by RT-PCR. The presence of TTP in the immunoprecipitates was detected by Western blotting with anti-TTP antibody. B and C, RNA EMSA. An RNA EMSA was performed by mixing cytoplasmic extracts containing 4 μg of total protein from pcDNA6/V5-TTP-transfected 293-EBNA cells (B) or A549 cells (C) with 20 fmol of biotinylated wild-type (Wt; ARE-3w/4w) or mutant (Mut; ARE-3m/4w) probe. Anti-V5 (B), anti-TTP (C), or control antibody (Ab) was added to the reaction mixtures. The binding reactions were then separated by electrophoresis on a 5% polyacrylamide gel under nondenaturing conditions. Arrows indicate the position of the TTP-containing band.

    Article Snippet: The biotinylated RNA probes for the wild type (LATS2-ARE-w3/w4, 5′-UUCAAAUUAGUAUGAUUCCUAUUUAAAGUGAUUUAUAUUUGAGUAAAAAGUUCAA-3′) and mutant (LATS2-ARE-m3/w4, 5′-UUCAAAUUAGUAUGAUUCCUAGCAAAGUGAUUUAUUUGAGUAAAAAGUUCAA-3′) were synthesized by Samchully Pharmaceutical Co., Ltd. (Seoul).

    Techniques: Immunoprecipitation, Luciferase, Construct, Mutagenesis, Negative Control, Transfection, Amplification, Reverse Transcription Polymerase Chain Reaction, Western Blot, Binding Assay, Electrophoresis